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D3: Large scale protein production of functional proteins – 17001

Preparation of Biomolecular Complexes for Structural Biology

(2 hrs/week; 2 CP; Fall 2020)

Sebastian Hiller, Timm Maier

Large amounts of functionally and structurally intact protein are essential for structural biology and many other fields of biochemistry and biophysics. Such amounts are rarely available from native material. This lecture series will focus on the various approaches for large-scale protein production in prokaryotic and eukaryotic cell lines as well as in vitro systems, their respective advantages and disadvantages, strategies to overcome the various problems inherent to the systems and on the functional recovery of the proteins themselves.

Confirmed Speakers

  • Dr. Alvar Gossert (Head of BioNMR Platform, ETH Zürich): "Producing isotope labeled proteins for NMR studies of pharmaceutically relevant targets"
  • Prof. Markus Seeger (Institut für Medizinische Mikrobiologie, Universität Zürich): "Sybody selections - from library design to the conformational trapping of membrane transporters"
  • Prof. Florian Schur (Institute of Science and Technology Austria): "Principles of in-situ structural biology using cryo-electron tomography / From Viruses to Cells –Toward high-resolution cellular structural biology"
  • Dr. Michael Hennig (CEO of LeadXpro AG, Villigen, Switzerland): "Enabling structure based drug discovery on membrane protein drug targets"
  • Prof. Maria Hondele (Biozentrum, University of Basel): "Molecular principles of liquid-liquid phase separation and purification of aggregation-prone proteins"
  • Dr. Jonas Barandun (The Laboratory for Molecular Infection Medicine Sweden, Umeå University): "Analysis of ribosome assembly and hibernation with structural biology techniques"
  • Dr. Guillaume Mas (Biozentrum, University of Basel): on methyl labelling for NMR
  • Dr. Thomas Braun (C-CINA, University of Basel): "Microfluidic Sample Preparation for Electron Microscopy"
  • Prof. Lisa Jones (Department of Pharmaceutical Sciences, University of Maryland, USA): on footprinting for studying protein structure in isolation, and in cells up to proteome scale
  • Prof. Imre Berger (School of Biochemistry, University of Bristol, UK): "The MultiBac BEVS Platform in the Bristol COVID Response"
  • Dr. Ben Engel (Helmholtz Pioneer Campus, Munich): on cryo electron tomography
  • Prof. Florian Seebeck (Department of Chemistry, University of Basel)

Organisational notes

Due to the COVID-19 pandemic, this lecture series is held in an interactive online format. Lectures are pre-recorded and uploaded to the SWITCHtube server (for links to the individual lectures, see below). Students watch the lectures and then meet for a moderated discussion on the lecture content at the dates and times listed in the table below. In the discussion, the students will formulate questions and feedback, record it and send it back to the lecturer. In some cases, the lecturer will join the discussion online. The lecture series closes with student's chalk talks, which are 10-minute presentations on a chosen topic. 


Online meetings and student chalk talks are scheduled on Thursday 13:15 – 15:00


Introduction (Zoom live session, ca. 30 minutes)


Lecture 1 (Zoom live session on Principles of Protein Preparation, Timm Maier)


Lecture 2 + 3 (available online for individual study)


13:15 Online meeting to discuss lectures 1-3


Lecture 4 (available online for individual study)


Lecture 5 + 6 (available online for individual study)


13:15 Online meeting to discuss lectures 4-6


Lecture 7 (available online for individual study)


Lecture 8 + 9 (available online for individual study)


13:15 Online meeting to discuss lectures 7-9


Lecture 11 + 12 (available online for individual study)


Student chalk talks


13:15 Online meeting to discuss lectures 10-12


Student chalk talks