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LEICA POINT SCANNING CONFOCAL “SP5-II-MATRIX“
LOCATION: Room 07.079
SPECIFICATIONS:
- Imaging: Confocal for live imaging; FRAP
- Stand: Leica DMI 6000B with adaptive focus
- XY-stage: motorized
- Equipped with 2 HyD
- Fully equipped for live imaging, temperature and CO2 controlled
- Conventional scanner and resonant (ultra-fast, for reduced photodamage) scanner
- AOBS serves as a tunable dichroic for all lasers
- Software: LAS AF version 2.6.0.7266
OBJECTIVES | |||||
Objective | Magnification / NA | Medium | Availability | AFC | Working Distance |
HC PL Fluotar | 10x / 0.30 | air | Yes | Yes | 11 mm |
HCX IRAPO L | 25x / 0.95 | water | Yes | No | 2.40 mm |
HCX PLAN APO CS | 40x / 1.10 | water | Yes | No | 0.63 mm |
HCX PLAN APO | 63x / 1.20 | water | Yes | No | 0.22 mm |
HCX PLAN APO lambda blue | 63x / 1.40-0.60 | oil | Yes | Yes | 0.10 mm |
HCX PLAN APO lambda blue | 40x / 1.25 | oil | Yes | Yes | 0.10 mm |
HCX PLAN APO CS | 20x / 0.70 | air | On Demand | Yes | 0.59 mm |
LASER LINES | |||
Wavelengths (nm) | Availability | Type | Some dyes and use |
405 | YES | Diode laser, 50mW | Alexa405, DAPI |
458 | YES | Argon laser, 5mW | CFP |
476 | YES | Argon laser, 5mW | |
488 | YES | Argon laser, 20mW | GFP, Alexa488, Cy2 |
496 | YES | Argon laser, 5mW | |
514 | YES | Argon laser, 20mW | YFP |
561 | YES | DPSS laser, 20mW | RedFPs, Alexa568 |
633 | YES | HeNe laser, 10mW | DRAQ5, Cy5, Alexa648 |