Main Content
LOCATION: Room 07.085
SYSTEM SPECIFICATIONS:
- Imaging: Super-resolution (fixed and live), structured illumination (3D-SIM), Ring TIRF (works with PALM or TIRF techniques)
- SIM resolution xy 100-160 nm; z 280-350 nm – Pixel resolution after reconstruction = 41 nm
- Stand: DeltaVision OMX Optical Microscope (version 4)
- XYZ-stage: motorized with 25 mm travel maximum. Piezo controlled Z axis system
- UltimateFocus Hardware Autofocus module with FocusAssist
- Fully equipped for live imaging, temperature and CO2 controlled
- Software: DeltaVision OMX softWoRx
CAMERA SPECIFICATIONS:
- 4 liquid cooled sCMOS cameras
- Effective number of pixels: 512 x 512 for SIM imaging, 1024 x 1024 for widefield
- Resolution: 0.08 μm pixel size
- Total chip size: 5.5 Megapixels
- Max frame rate: 400 frames/second
- Rolling Shutter and Global Shutter modes
- Dynamic range: 15 bit readout
- Quantum efficiency (peak): ~ 65%
OBJECTIVES | ||||
Objective | Magnification / NA | Medium | Use for | Working Distance |
U-PLAN S-APO | 100x / 1.40 | oil | Widefield + DIC | 0.12 mm |
Plan Apo N | 60x / 1.42 | oil | 3D-SIM | 0.15 mm |
APON | 60x / 1.49 | oil | TIRF | 0.1 mm |
UPLS Apo | 60x / 1.20 | water | (to be delivered) | 0.28 mm |
LASER LINES (Solid state illumination) | |||
Wavelengths (nm) | Availability | Type | Some dyes and use |
405 | YES | InsightSSI | DAPI |
445 | YES | InsightSSI | CFP |
488 | YES | InsightSSI | GFP, Alexa488, Cy2 |
514 | YES | InsightSSI | YFP |
568 | YES | InsightSSI | RedFPs, Alexa568 |
642 | YES | InsightSSI | DRAQ5, Cy5, Alexa647 |
EMISSION FILTERS | ||||
Fluorochromes | Availability | Bandpass | Emission | Drawer |
DAPI | YES | Single-band | 419 - 451 | BGR |
CFP | YES | Single-band | 456 - 495 | CYR |
GFP / FITC | YES | Single-band | 504-552 | BGR |
YFP | YES | Single-band | 530-552 | CYR |
mCherry / Alexa568 | YES | Single-band | 590-628 | BGR / CYR |
Cy5 / Alexa647 | YES | Single-band | 663-703 | BGR |