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Olympus SpinSR (CSU-W1)

LOCATION: Room 07.081

SYSTEM SPECIFICATIONS:

  • Imaging: Fast imaging with low phototoxicity;
  • Stand: Olympus IX83
  • XY-stage: motorized
  • Temperature and CO2 incubation
  • Software: cellSens
  • Yokogawa CSU-W1 confocal scan head (50um disk)

CAMERA SPECIFICATIONS:

  • Model: Hamamatsu ORCA-Fusion, sCMOS
  • Effective number of pixels: 2304 x 2304
  • Cell size: 6.5µm x 6.5µm
  • Effective Area: 14.976mm x 14.976mm
  • Max frame rate: 100 frames/second
  • Dynamic range: 16 bit
  • Quantum efficiency (peak): > 80% (@550nm)
  • Read out noise: 0.7 - 1.4 electrons (depending on fps)

OBJECTIVES

Objective

Magnification / NA

Medium

Contrast

Working Distance

UPL X APO

4x / 0.16

air

-

13 mm

UPL X APO 

10x / 0.4

air

-

3.1 mm

UPL S APO

30x / 1.05

silicone

-

0.8 mm

UPL APO

60x / 1.2

water

-

0.28 mm

UPL APO

60x / 1.5

oil

-

0.11 mm

UPL APO

100x / 1.5

oil

-

0.12 mm

LASER LINES

FIXED wavelengths

Availability

Laser type

Some dyes and use

405nm

YES

diode laser

DAPI

488nm

YES

diode laser

GFP, Alexa488

561nm

YES

diode laser

Cy3, Alexa568

640nm

YES

diode laser

Cy5, Alexa647

EMISSION FILTERS in front of camera 1

Fluorochromes

Availability

Bandpass

Transmission (nm)

DAPI

YES

single BP

422-472

GFP, Alexa488

YES

single BP

500-550

RFP, Alexa568

YES

single BP

580-653

Cy5, Alexa647

YES

single BP

665-705

Beam splitter when using dual camera mode: Long pass 561nm

EMISSION FILTERS in front of camera 2

Fluorochromes

Availability

Bandpass

Transmission (nm)

DAPI

YES

single BP

422-472

GFP, Alexa488

YES

single BP

500-550