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Olympus SpinD (CSU-W1)

LOCATION: Room 07.079

SYSTEM SPECIFICATIONS:

  • Imaging: Fast imaging with low phototoxicity;
  • Stand: Olympus IX83
  • XY-stage: motorized
  • Software: cellSens
  • Yokogawa CSU-W1 confocal scan head (50um disk)

CAMERA SPECIFICATIONS:

  • Model: Hamamatsu ORCA-Fusion, sCMOS
  • Effective number of pixels: 2304 x 2304
  • Cell size: 6.5µm x 6.5µm
  • Effective Area: 14.976mm x 14.976mm
  • Max frame rate: 100 frames/second
  • Dynamic range: 16 bit
  • Quantum efficiency (peak): > 80% (@550nm)
  • Read out noise: 0.7 - 1.4 electrons (depending on fps)

OBJECTIVES

Objective

Magnification / NA

Medium

Contrast

Working Distance

UPL X APO

4x / 0.16

air

-

13 mm

UPL X APO 

10x / 0.4

air

-

3.1 mm

UPL S APO

30x / 1.05

silicone

-

0.8 mm

UPL APO

60x / 1.5

oil

-

0.11 mm

UPL APO

100x / 1.5

oil

-

0.12 mm

LASER LINES

FIXED wavelengths

Availability

Laser type

Some dyes and use

405nm

YES

diode laser

-

488nm 

YES

diode laser

-

561nm

YES

diode laser

-

640nm

YES

diode laser

-

EMISSION FILTERS in front of camera 1

Fluorochromes

Availability

Bandpass

Transmission (nm)

DAPI

YES

single BP

422-472

GFP, Alexa488

YES

single BP

500-550

RFP, Alexa568

YES

single BP

580-653

Cy5, Alexa647

YES

single BP

665-705

Beam splitter when using dual camera mode: Long pass 561nm

EMISSION FILTERS in front of camera 2

Fluorochromes

Availability

Bandpass

Transmission (nm)

DAPI

YES

single BP

422-472

GFP, Alexa488

YES

single BP

500-550