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ZEISS lightsheet microscope “Lightsheet 7“

LOCATION: Room 07.092

SPECIFICATIONS:

  • Imaging: Lightsheet for live & fixed large samples
  • Stand: Zeiss Z1 lightsheet microscope
  • XYZ & rotation-stage: motorized
  • Fully equipped for live-imaging, Temperature and CO2 controlled
  • Integrated multi-view reconstruction, deconvolution
  • Software: Zen 2010 for light sheet microscopy

CAMERA SPECIFICATIONS:

  • 2 liquid cooled sCMOS cameras, pco.EDGE
  • Effective number of pixels: 1920 x 1920
  • Resolution: 0.23 μm pixel size
  • Max frame rate: 30 frames/second
  • Dynamic range: 16 bit readout
  • dynamic range of 36 000 : 1
  • extreme low readout noise of 0.8 e- med
  • quantum efficiency above 70%

OBJECTIVES

Objective

Magnification / NA

Medium

Chamber/RI

Working Distance

FLUAR

2.5x / 0.12

air

clearing

-

PLAN NeoFluar

5x / 0.16

air

water & clearing

1.33 - 1.58

5.1 mm

PLAN APO

20x / 1.0

water dipping

water 1.33

2.4 mm

PLAN APO

40x / 1.0

water dipping

water 1.33

2.5 mm

PLAN APO

63x / 1.0

water dipping

water 1.33

2.1 mm

PLAN APO

20x / 1.0

clarity dipping

clarity 1.46

5.6 mm

PLAN APO

20x / 1.0

ECi dipping

ECi 1.48 - 1.58

5.6 mm

LASER LINES

FIXED wavelengths

Laser type

Some dyes and use

405nm

solid state, 5mW

DAPI

488nm

solid state, 10mW

GFP, Alexa488, Cy2

561nm

solid state, 10mW

RedFPs, Alexa568, Cy3

640nm

solid state, 5mW

DRAQ5, Cy5, Alexa647

FILTER SETS

Fluorochromes

Wavelengths (nm)

Mirror (nm)

Filter 1 (nm)

Filter 2 (nm)

Blue / Green

405 / 488

LP 510

420 - 470

525 - 545

Green / Red

488 / 561

LP 560

510-540

505 - 545

Green / Red

488 / 561

LP560

505-545

LP 585

Green / Far Red

488 / 638

LP 560

505 - 545

LP 660