Main Content
Benchtop mesoSPIM (mesoscale selective plane illumination microscopy)
LOCATION: Room 07.092
SPECIFICATIONS:
- XY-stage: motorized
- Software: mesosPIM-control
- Camera: Photometrics IRIS 14
- 25 mm camera sensor FOV (diagonal)
- 4.25 um pixel size
- 15 MP pixels / image
OBJECTIVES |
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Objective | Magnification / NA | Medium | Working Distance |
Plan Apo | air | 3.4 mm |
ILLUMINATION |
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Wavelengths (power) | Availability | Type | Some dyes and use |
488 (100 mW) | YES | Solid State Laser | mEGFP, Alexa 488 |
561 (100 mW) | YES | Solid State Laser | Tdtomato, mCherry, Alexa 568 |
638 (180 mW) | YES | Solid State Laser | Alexa 647, Alexa 660 |
750 | NO | Solid State Laser | Alexa 750, Alexa 790 |
EMISSION FILTERS |
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|---|---|---|---|
| dyes | em filter | filter wheel position | AHF number |
| A488, mEGFP | 520/35 Brightline HC | 1 | F37-520 |
| A568, Tdtomato, mCherry | 595/50 ET Bandpass | 2 | F47-595 |
| A647, A660 | 679/41 Brightline HC | 3 | F37-679 |
| For light sheet alignment | EMPTY | 4 | - |
| all above + A750, A790 | 432/515/595/681/809 | 5 | F67-050 |