Navigation mit Access Keys

Main Content

Nikon CSU-W1

LOCATION: Room 09.169 (BSL2)

SYSTEM SPECIFICATIONS:

  • Imaging: Fast imaging with low phototoxicity
  • Stand: Nikon Ti2 with perfect focus system (PFS)
  • XY-stage: motorized
  • Temperature and CO2 incubation
  • Software: NIS elements with JOBS
  • Yokogawa CSU-W1 confocal scan head (50um disk)

CAMERA SPECIFICATIONS:

  • Model: 2x Hamamatsu ORCA-Fusion, sCMOS
  • Effective number of pixels: 2304 x 2304
  • Cell size: 6.5µm x 6.5µm
  • Effective Area: 14.976mm x 14.976mm
  • Max frame rate: 100 frames/second
  • Dynamic range: 16 bit
  • Quantum efficiency (peak): > 80% (@550nm)
  • Read out noise: 0.7 - 1.4 electrons (depending on fps)

OBJECTIVES

Objective

Magnification / NA

Medium

Contrast

Working Distance

CFI Plan Fluor

4x / 0.13

air

-

17.2 mm

CFP Plan Apo Lambda

10x / 0.45

air

-

4 mm

CFI Plan Apo DM Lambda

20x / 0.75

air

Ph2

1 mm

CFI Plan Apo Lambda S

25x / 1.05

silicon

DIC

0.55 mm

CFI Apo LWD Lambda S

40x / 1.15

water

-

0.61 - 0.59 mm

CFI Plan Apo Lambda

100x / 1.45

oil

DIC

0.13 mm

ILLUMINATION

Wavelengths (nm)

Availability

Type

Some dyes and use

405

YES

Diode laser

Alexa405, DAPI

488

YES

Diode laser

Alexa488, GFP

561

YES

Diode laser

Alexa568, RFP

640

YES

Diode laser

Alexa647, Cy5

395/25

YES

Solid state light

DAPI

440/20

YES

Solid state light

CFP

470/24

YES

Solid state light

GFP, Alexa488, FITC

508/25

YES

Solid state light

YFP

555/15

YES

Solid state light

TRITC, Cy3

640/30

YES

Solid state light

Alexa647, Cy5

DICHROIC MIRROR

Fluorochromes

Availability

Mirror (nm)

Transmission (nm)

DAPI

YES

400-415

422-473

GFP, Alexa488

YES

483-497

503-545

RFP, Alexa568

YES

561-568

586-620

Cy5, Alexa647

YES

638-647

665-750

EMISSION FILTERS in front of the camera

Fluorochromes

Availability

Bandpass

Transmission (nm)

DAPI

YES

single BP

417-477

GFP, Alexa488

YES

single BP

510-540

RFP, Alexa568

YES

single BP

574-626

Cy5, Alexa647

YES

single BP

671-745