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FEI MORE

LOCATION: Room 07.090

SPECIFICATIONS:

  • Imaging: very fast and stable inverted widefield microscope, FRAP, TIRF
  • Stand: MORE frame and enclosure
  • XY-stage: motorized
  • Fully equipped for live imaging, temperature and CO2 controlled
  • Software: Live Acquisition 2.5
  • Camera: Hamamatsu ORCA flash 4.0 cooled sCMOS
    • Effective number of pixels: 2048 x 2048
    • Cell size: 6.5µm x 6.5µm
    • Effective Area: 13.312mm x 13.312mm
    • Maximum frame rate: 30fps
    • Dynamic range: 16-bit
    • Quantum efficiency (peak): > 70%
    • Read out noise: 1.9 electrons rms

OBJECTIVES

Objective

Magnification / NA

Medium

Contrast

Working Distance

U Plan S Apo

10x / 0.40

air

PH2

3.1 mm

U Plan S Apo

40x / 0.95

air

PH2

0.38 mm

TIRF APON

60x / 1.49

oil

-

0.1 mm

U Plan S Apo

100x / 1.4

oil

-

0.13 mm

ILLUMINATION

Wavelengths (nm)

Availability

Type

Some dyes and use

405

YES

Solid state laser

DAPI

488

YES

Solid state laser

GFP, Alexa488, Cy2, FITC

561

YES

Solid state laser

TRITC

640

YES

Solid state laser

DRAQ5, Cy5, Alexa647

395/25

YES

Solid state light

DAPI

434/17

YES

Solid state light

CFP

470/24

YES

Solid state light

GFP, Alexa488, Cy2, FITC

510/10

YES

Solid state light

YFP

550/15

YES

Solid state light

TRITC, Cy3

575/25

YES

Solid state light

On demand (mCherry)

631/28

YES

Solid state light

DRAQ5, Cy5, Alexa647

470

YES

LED (Trans)

Transillumination

540

YES

LED (Trans)

Transillumination

630

YES

LED (Trans)

Transillumination

BANDPASS FILTERS (Emitters)

Fluorochromes

Availability

Bandpass

Transmission (nm)

DAPI

YES

single BP

425-443

GFP, Alexa488

YES

single BP

507-537

RFP, Alexa568

YES

single BP

665-705

CFP / YFP

YES

dual BP

457-471 / 536-558

DAPI / GFP / Cy3 / Cy5

YES

quad BP (LED)

442-442 / 506-524 / 585-605 / 692-768

DAPI / FITC / TRITC / Cy5

YES

qud BP (Lasers)

370-410 / 491-552 / 590-628 / 663-703