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LEICA POINT SCANNING CONFOCAL “SP5-II-MATRIX“

LOCATION: Room 07.079

SPECIFICATIONS:

  • Imaging: Confocal for live imaging; FRAP
  • Stand: Leica DMI 6000B with adaptive focus
  • XY-stage: motorized
  • Equipped with 2 HyD
  • Fully equipped for live imaging, temperature and CO2 controlled
  • Conventional scanner and resonant (ultra-fast, for reduced photodamage) scanner
  • AOBS serves as a tunable dichroic for all lasers
  • Software: LAS AF version 2.6.0.7266

OBJECTIVES

Objective

Magnification / NA

Medium

Availability

AFC

Working Distance

HC PL Fluotar

10x / 0.30

air

Yes

Yes

11 mm

HCX IRAPO L

25x / 0.95

water

Yes

No

2.40 mm

HCX PLAN APO CS

40x / 1.10

water

Yes

No

0.63 mm

HCX PLAN APO

63x / 1.20

water

Yes

No

0.22 mm

HCX PLAN APO lambda blue

63x / 1.40-0.60

oil

Yes

Yes

0.10 mm

HCX PLAN APO lambda blue

40x / 1.25

oil

Yes

Yes

0.10 mm

HCX PLAN APO CS

20x / 0.70

air

On Demand

Yes

0.59 mm

LASER LINES

Wavelengths (nm)

Availability

Type

Some dyes and use

405

YES

Diode laser, 50mW

Alexa405, DAPI

458

YES

Argon laser, 5mW

CFP

476

YES

Argon laser, 5mW

488

YES

Argon laser, 20mW

GFP, Alexa488, Cy2

496

YES

Argon laser, 5mW

514

YES

Argon laser, 20mW

YFP

561

YES

DPSS laser, 20mW

RedFPs, Alexa568

633

YES

HeNe laser, 10mW

DRAQ5, Cy5, Alexa648